Climate has been changing rapidly in the last decades, causing shifts in plant distribution. Thus, it is essential to reveal if some populations of woody plants might be more drought-resistant thanks to differing stomatal characteristics. The most crucial function of stomata is the regulation of water losses from a plant and the inflow of CO2. The objective of this work is to assess differences in the size of needles and stomata and in the stomatal density. The size of stomata can affect the speed of the response during the drought stress event. We measured 960 needles sampled from five provenances (Austrian, two Slovak and two Polish ones) originating from different altitudes. We assessed three needle-year-classes and their stomatal traits. We found significant differences among provenances for all tested features and also, we found significant differences among years (except for the stomal length). The interaction provenance × year was statistically significant for all assessed parameters. Three-year-old needles differed from overall average values and populations originating from the most extreme localities showed minimal response to the drought occurring within that particular year.