The diploma thesis deals with the use of modern analytical methods for the identification of hazardous substances in Fire and Rescue Services. The aim of the diploma thesis was to characterize and analyze the use of modern analytical methods for the identification of hazardous substances in Fire and Rescue Services . To evaluate the measurements of selected tested samples of chemical substances using a spectrum analyzer in the form of training of Fire and Rescue Services members at a selected fire station.In this work, 14 samples of chemical substances were tested, included flammable liquids, oxidizing liquids, acids, and bases. The samples were determined by Raman spectroscopy. A hand-held Raman spectrometer Progeny ResQ was used. Sampling and scanning of samples were performed by members in chemical suits.The success rate of the research was 50%. Seven samples of substances were identical to the substances in the spectrometer library. The Progeny ResQ spectrometer was unable to determine substances with the simplest chemical composition. However, the spectrometer was also able to identify a small amount of a dangerous substance. The fabric sample could be scanned through a plastic sample box, a glass vial, and a resealable bag.The spectrometer is durable, waterproof, and easy to set up. Due to its low weight and small dimensions, it is used in interventions with the suspected occurrence of hazardous substances, so various portable chemical laboratories are not needed. Another advantage of using Progeny ResQ is the identification of a wide range of unknown substances. The time interval for scanning substances is always the same and does not depend on the chemical composition of the substances.