ŠKOLEK Matúš: Changes of physico-chemical and microbial attributes of topsoil in pine forests of different age. (Bachelor's thesis) Supervisor - doc. Ing. Erika Gömöryová, CSc., Technical university in Zvolen, Faculty of forestry, Department of Natural Environment. 2019, 48 p.In our bachelor thesis, we focused on the comparation of physico-chemical and microbial attributes in pine forests of different age. These forests differ in vegetation cover and also microclimate. We collected soil samples at plots in seven forest stands in 2018. These locations were situated on sands of Borska lowland. Then we took samples to the laboratory, where in soil samples we determined : soil moisture, soil reaction and content of total N, C, S, exchangeable K, Mg, Ca and plant available P. in soil. Equally, we detected information about C/N ratio. Among the microbial characteristics, we exposed N-mineralization, basal respiration, microbial biomass carbon, richness and diversity of soil microbial functional groups. Among physico-chemical attributes, we found out the biggest differences between stands of different age in soil moisture and soil reaction. There were significant differences also in the content of P and S. The older forests exhibited higher C/N ratio and rate of N-mineralization. Vice-versa, a higher rate of basal respiration was found in younger forests. Other microbial attributes did not show significant differences.