In the past, spruce stands were often planted in inappropriate and unnatural habitats for this woody species. The reason for this is the popularity of spruce timber today. The unsuitability of planting spruce monocultures manifests itself in the disintegration of spruce cultures, therefore, we consider it important to determine their origin and consequently their suitability in individual provenance of spruce in Slovakia.We used mtDNA as the non-recombining DNA that is in Pinaceae is maternally inherited and as such used for analysis of gene flow via seed, especially for phylogenetic studies and analysis of different glacial refugia origin. Populations from different glacial refugia can have different adaptive properties as a result of drift or adaptations. The aim of the work was to analyse the seed sources and to determine their mitochondrial haplotypes and to investigate whether they correspond to mitotypes occurring in Central Europe and thus to infer that they are of autochtonous and coming from regions that are suitable as sources of forest reproductive material for the species , at least on a rough scale , that correspond to maternal lineage. We analysed 12 seed sources. We extracted DNA and used simple mitochondrial marker to infer whether mitochondrial haplotypes are the ones occurring in Central Europe.