Interest in replacing the mammalian model for drug testing is still very actual. One of the options is the use of avian chorioallantoic membrane (CAM), which has been used for the study of e.g. tumor angiogenesis or antivascular therapy. In our study we used less common model of Japanese quail (Coturnix japonica) CAM. The objectives of this study were to assess the suitability of experimental quail CAM model for the study of photodynamic diagnosis and therapy of tumors (PDD, PDT), monitoring photodynamic effect of hypericin (Hyp) on the CAM vasculature, photodynamic diagnosis of cancer on CAM, including the testing of suitable delivery system. And finelly monitoring of invasiveness of hypoxic tumor cells and modulation of tumor hypoxia marker carbonic anhydrase IX (CA IX).Our experimental results show that photoactivated Hyp had destructive effect on the vasculature, including the blood vessels photothrombosis. To increase the selectivity and efficiency of Hyp, we examined LDL delivery system (low density lipoprotein). Our results show that the tumor:normal tissue difference in fluorescence intensity is higher for Hyp:LDL complex and represents a suitable formulation to optimize PDD. An important results of our work is the role of PG domain of CA IX, blocking which resulted in decreased migration of tumor cells into CAM. CAM seems to be an excellent model for experimental oncology with diagnostic and therapeutic potential.