(Bc. Špirko, Samuel: The using of different types of substrates by growing spruce seedlings, (Diploma thesis) Technical university in Zvolen, Faculty of Forestry LF 104162-17193, 2020 50s.)The main aim of this diploma thesis was to analyze the results of spruce seedlings grown on different types of substrates, by using Duneman´s method . The research was made on one of the seedbed in forest nursery Semenište, belongs to LS Ľubica, o. z. Kežmarok, VLM SR. Spruce seedlings were grown in two separate experiments. In the first experiment, named Spruce 1., the results of seedlings grown on three types of substrate after the second growing season were evaluated, in the second experiment, named Spruce 2., the results of seedlings grown on 4 types of substartes after the first growing season were evaluated. In the Spruce 1 experiment mineral soil, compost and peat were selected as substrates. In the Spruce 2 experiment were selected the same substrates as in the Spruce 1 experiment plus the combination of compost and peat was selected as a fourteh substrate. There was a same area defined in each experiment. Individual substrate areas were apportioned into 3 repetiton. From the each repetiton in each substrate 10 samples of subjects were taken, it means 30 samples of each substrate were taken. Overall there were 90 samples of Spruce 1 and 120 samples of Spruce 2 taken. Afterwards biometric characteristic were determined: the lenght of aboveground part, the lenght of root part, and the thickness of the root neck. After drying the weight of the dried aboveground and root part was also determined. These characteristics were evaluated by one-factor analysis of variance using repeats at a significance level of α= 0,05. The best result were achieved in seedlings grown on peat substrate, in both experiments. Positive results were also achieved on the substrate peat and compost, in which the seedlings reached double values as on the substrate mineral soil and compost.