The thesis is focused on the design and theoretical-experimental verification of thermal-technical characteristics and moisture content of wood-based prefabricated panel walls using natural insulations. The theoretical part of the thesis deals with the analysis of the wood-based prefabricated structural system, the concept of design of low-energy buildings and the functions of the various layers of packaging structures in terms of diffusion of water vapour. The research part deals with the theoretical analysis and experimental verification of the thermal-technical characteristics of two types of perimeter walls – with a paper-based vapour barrier and with an OSB3 (Oriented Strand Board) plate. The main aim is to determine the moisture content of materials and structures during the year in the measured period and at the same time to confront the theoretical and experimental results. The results show compliance with the present requirements of STN 73 0540-2 as well as the standard operating state of moisture of materials incorporated. The selection and application of the proposed structures for selected architectural solutions of a single-storey house in order to develop the project of energy efficiency of the building (EEB) and classification into the energy class in accordance with the Slovak legislation are given in the following part of the thesis is. The proper construction and technical solution of the building and the analysis of the EEB follows the current requirements for the EEB.