Global environmental contamination currently ranks among one of the most important problems. The impact of heavy metals on the environment is highlighted by their characteristic property and that is non-degradability. One of the environmental burdens that poses an issue for the environment is the sludge bed Kaligrund, located at Slovinky village in the East Slovak. This village is marked by long-term mining. One way to solve such an environmental problem is bioremediation. For this purpose, the master thesis is focused on the isolation, identification, and resistance of bacteria in mining waters at the sludge bed Kaligrund. The sludge bed consists of deposited sludge from copper and iron mining, the upper part of sludge bed is covered with slag from nearby of company "Kovohuty". For identification microorganisms, we used cultivation methods, microscopic methods. Pure cultures were analyzed by a high reliability method of mass spectrometry MALDI-TOF. It was detected the presence of bacteria as Shewanella putrefaciens, which can use various compounds as electron acceptors, for example oxygen, iron, manganese, nitrates, nitrites, uranium, fumarates, has been found. The tested bacteria can grow in a heavy metal environment, we can assume that this type of bacteria could be potentially applicable in the removal of toxic elements from the environment, in bioremediation.