This work is focused on the changes of physico-chemical and microbial properties of surface organic layer (SOL) in connection with the age of pine forests. In addition to the age, the forests differed in the herb layer and microclimate. In 2018, we took three samples from SOL in seven different age classes of stands. The forests were located in the military area on the eolic sands of Záhorská nížina between the villages Lakšárska Nová Ves and Plavecký Mikuláš. Subsequently, we determined soil moisture, pH, concentrations of biogenic elements and C / N ratio in the laboratory. Among the microbial characteristics, we determined microbial carbon, richness and diversity of microorganisms, basal respiration and N-mineralization. Evaluating physico-chemical properties, we found a decreasing pH value with stand age. Most of the elements had the highest concentrations in the 0-10 year old forests. Regarding the microbial attributes, we found the highest N-mineralization and basal respiration in the 20-40 year old forest. Other microbial characteristics did not differ significantly.