Mining influenced water is often a source of environmental contamination. In the Central Slovak neovolcanics field, there are several risk sources that have been the subject of the research. Based on the physico-chemical analysis, the mine drainage from the Voznická dedičná štôlňa adit, which is a source of Mn and Zn contamination, appears to be the riskiest for the environment. Drainage water from the tailings pond in Hodruša-Hámre village affects the pH and concentrations of sulphates and other hazardous elements in the local surface stream. By microbiological analysis of mine drainage, we obtained bacterial isolates, the ability of which to be resistant to risk elements was tested. Isolate 4.2 was identified as Serratia liquefaciens as one of the most resistant. In some isolates, their ability to sorption Ni, Pb, Fe, Mo and Hg from the medium was confirmed. Several isolates were multimetal-resistant and were able to biosorb more metals from the medium – Providencia rettgeri, Lysinibacillus fusiformis and Bacillus cereus. These results indicate their potential for bioremediation processes.