Stress can be dangerous if it affects an organism for a long time. After planting an area, the planting material is exposed to various forms of stress. Drought stress is increasingly occurring in connection with climate change and gradual warming. The bachelor thesis deals with the monitoring of simulated drought stress and its effect on spruce seedlings (Picea Abies (L.) Karst). The theoretical part contains information and knowledge about drought stress and possible eradication of drought stress in forest trees. One possibility is to apply commercial products that potentially eliminate stress. The practical part presents an experiment that evaluates the impact of drought stress simulated by different irrigation regimes: without irrigation, half, periodic and full irrigation. Agrisorb hydrogel and Ectovit mycorrhizal preparations were used for full irrigation during the development of two-year-old spruce seedlings (1 + k1). The preparations were applied when nursing 1-year-old seedlings into packages in the spring and the stress was simulated in the following growing season. Chlorophyll fluorescence was measured before the end of the growing season, root neck thickness, above ground part weight, root system weight, above ground weight to seedling root weight ratio, number of short roots, proportion of mycorrhizal in the total number of roots, and also root morphology were measured. Mycorrhizal morphotypes were distinguished and described.