On 26th June in 2020, monitoring was carried out at 3 selected localities in the Danube lowland in the districts of Komárno and Dunajská Streda. The aim of our work was to determine the current state of populations of the European mudminnow (Umbra krameri) in the studied localities and compare it with data from previous monitorings. We also assessed negative factors in localities with a proposal for management measures to improve the state of populations. Sampling was performed by wading, using a battery-powered electrochocker. We recorded 10 species of fish at the examined localities, of which 3 species were invasive - Black bullhead catfish (Ameiurus melas), Topmouth gudgeon (Pseudorasbora parva) and Pumpkinseed (Lepomis gibbosus). We confirmed the presence of the European mudminnow only in the locality Čiližsky brook, where it occurred together with the invasive species of Pumpkinseed. We did not confirm the occurrence of the European mudminnow in the localities of the Hamský canal, but we found a very large population of Black bullhead catfish and Topmouth gudgeon, which will probably be the main cause of the absence of the European mudminnow in the localities. The report on the status of habitats and species of European importance in the Slovak Republic for the period 2013-2018, prepared by the State Nature Conservacy of the Slovak Republic, assessed the abundance of European mudminnow in the Pannonian biogeographical region of Slovakia as low and overall assessment of conservation status is unfavorable.