The bachelor thesis are focused on the current development of molecular methods, which enables us to examine organisms and their genomes on the higher number of genetic markers by next-generation sequencing. In the first part, we describe new methods that enabled a gradual transition from the conservation genetics to conservation genomics. The obtained results provide more detailed information about the genetic structure, genetic diversity and gene flow in wildlife populations. Next we describe concepts and terms commonly used in genomics. In the second part, we describe the basic assumption of genomic analysis, which is the correct design of the collection of invasive and non-invasive samples and their appropriate storage. In the third part, we describe the extraction method of high-quality non-fragmented genomic DNA. High qualitative demands on extracted DNA are often a limiting factor for the success of the study of the organism´s genome. In the next part, we describe the extraction protocol of DNA. In the results, we describe quantitative and qualitative parameters of the extracted DNA, i.e. its concentration and purity indicators.