Heavy metals are among the basic groups of substances that pollute the environment. Therefore, their impact on humans or, overall, the environment is currently a worldwide concern. The content of heavy metals in food but also in wine is the subject of many scientific research not only in the world but also in Slovakia. In this work we deal with complex analysis of heavy metals in wine. In the first chapter of the work we will get acquainted with wine and its chemical composition in general. Furthermore, we focus on the selected heavy metals, which are most commonly found in wines, and are: cadmium, lead, chromium, copper and nickel. There are several procedures for analyzing selected heavy metals in wines. We describe atomic absorption spectroscopy (AAS), which belongs to frequently used methods of research for determination of heavy metals content in wines. The second chapter presents the aim of the work. In the third chapter we present methods of analysis. We deal with two methods of atomic absorption spectroscopy, namely flame atomic absorption spectroscopy (FS AAS) and atomic absorption spectroscopy with graphite cuvette (GT AAS) for their basic characteristics. These are the two methods that are most commonly used today, and the results are provable. Heavy metals analyzed can also be used to detect GT AAS in small amounts of wine, which is very important as well as economical. It is very important to monitor the content of heavy metals in wines, as high-value wines such as cadmium, lead, nickel, zinc and copper (and others) can cause toxic effects on the living organism.