The urban vegetation has received great attention in the global climate change context in recent years. As the natural element of the city structure, vegetation can gradually improve environmental quality at minimum cost compared to all the technical options. Vegetation effects on improving the climate, produces oxygen, which has mainly phytoncide and regenerative impact, absorbs harmful contaminants from the air, reduces noise, dust and gaseous air pollution. By ionizing the air it positively affects his physical state, provides space and suitable conditions for recreation and recovery of people, compositionally and aesthetically completes the city, acts on the physiological and mental state of man. These effects will become evident only in case when the vegetation is systematically planned, cherished and regularly treated. Besides this solid treatment, achievement of these positive effects requires also modification of the methodology of vegetation regulation tools in spatial planning. In Slovakia we use a quantitative approach in the planning of city vegetation, which does not reflect the diversity of all the green surfaces in an urban environment. Nowadays this approach appears to be insufficient. The objective of this work is the proposal of the most optimal method for regulating the urban vegetation in zoning plans. This method shall take into consideration the qualitative aspects of green surfaces. The core of this work is to analyze the regulatory tools for city vegetation applied in Slovakia and the tools applied abroad. The objective of the practical research is the application of the analyzed regulatory tool on an existing ground and their subsequent mutual comparison. The final chapter defines the requirements that shall be implemented in the new methodology for regulation of green areas in spatial planning. Successful implementation of the reasearch findings can contribute to the ecological stability of the city.