The diploma thesis entitled "Leakage of a dangerous substance from a damaged tank on a motorway" deals with the analysis of the legislation on the transport of dangerous substances along the road, simulating the leakage of the selected dangerous substance toluene diisocyanate and the subsequent comprehensive intervention of the components of the Integrated Rescue System. The aim of our work is to model scenarios in the ALOHA program, to describe the activities of rescue teams and to offer proposals and solutions in the future that would increase the safety and health of the components involved in adverse events. The first chapter focuses on the current state of legislation. We systematically discuss individual pieces of legislation on chemicals and the transport of dangerous substances in national and international legislation. In the second chapter we characterize the selected dangerous substance. We describe its properties, shipping information and leakage to the environment. We also address the issue of an Integrated Rescue System associated with the operation of rescue teams at the scene. The result of our work is the creation of scenarios of an undesired event with the ALOHA simulation software on the motorway and the subsequent comprehensive intervention of components of the integrated rescue system. Work could serve as an alert against the undesirable effects of toluene diisocyanate in escape and subsequent intervention by rescue agents.