Lamination is one of the most commonly used methods of surface treatment of wood-based composites, such as particleboards, fibreboards etc. Lamination is typically carried out with decorative papers impregnated with amino thermosetting resins, mainly melamine-formaldehyde resin, urea-formaldehyde resin or their mixture. Conventional laminates are not bioactive and therfore no table to reduce or completely inhibit microbial growth when polluted with organic substances.Thus, the main aim of dissertation was to determine the effect of chosen biocidal additives, silver (Ag) nanoparticles and zinc oxide (ZnO) nanoparticles, added to melamine-formaldehyde (MF) for paper impregnation used for the surface treatment of particleboards by lamination against bacterial activity (Staphylococcus aureus, Escherichia coli) and moulds (Aspergillus niger, Penicillium brevicompactum). Furthermore, their effect on the mechanical, chemical and thermal resistance of the laminated surfaces of particleboards (L-PB) and their effect on laminate adhesion and color change after accelerated weathering was studied.The presence of Ag nanoparticles in surfaces of L-PB reduced activity of S. aureus on the surfaces about 43.9 % or about 28.8 % when contaminated by bacterial suspnsion of higher density. Ag nanoparticles were highly active against E. coli and were able to completely suppress or decrease the bacterial activity by 96.7 %. Likewise, Ag, nanoparticles in L-PB surfaces reduced the growth activity of P. brevicompactum on specimens surfaces, however they reduced the activity of A. niger only slightly. ZnO nanoparticles in L-PB´s surfaces reduced activity of S. aureus about 43.9 % or about 28,8 % when contaminated by bacterial suspnsion of higher density, and significantly the activity of E. coli about 76.6 % and 62.9 %. Similarly, ZnO nanoparticles in laminated surfaces were active aginast both moulds and were able to reduce their growth activity (GAM) value on the specimens surfaces from 4 to 2 in comparison to untreated laminate surfaces. By modifying MF resin with lower additions of Ag and ZnO nanoparticles, there was no significant negative effect on the resistance to cold liquids, dry heat and abrasion of surfaces of L-PBs, just as there was no reduction of laminate adhesion and no noticable color change after accelerated weathering of specimens while maintaining high antimicrobial properties.