In the first part of the bachelor thesis, we summarised available information from many literature sources about systematics, distribution, etology, breeding ecology, diet and conservation of two woodpecker species – Grey-headed and Green Woodpecker. The aim of the second part was determination of habitat preferences of Picus canus and Picus viridis. We realized 4 census along two point transects in the selected area during spring season 2017 – 2018. Six types of habitats were recognised: broadleaved forest, mixed forest, coniferous forest, urban biotop, grasslands and quarry. For assesing of relation between these habitat types and occurence of our investigated woodpecker species we used multiple linear regression and correlation analysis. Although our results were not statistically significant due to limited number of census points and low abundance of the species, which reaches often zero vallues, we found some relations between Picus sp. occurence and habitat. Occurence of Picus viridis correlated positively with presence of broadleaved forest and urban biotop and negatively with coniferous and mixed forest, grasslands and quarry. Occurence of Picus canus correlated positively with broadleaved forest and coniferous forest, negatively with grasslands, urban biotop and quarry and no correlation between its occurance and mixed forest was found. In our study area we found densities of Picus viridis and Picus canus 1,8 and 1,2 (average vallue from both yerars) bp per 100 ha respectively. Both Picus species are strongly specialised and therefore they are considered as environment quality bioindicators and belong to law protected animals, and knowing their habitat and ecology needs can be applied in these woodpeckers conservancy and in nature conservation as well, because they are also known as umbrella species. We also focused on winter food diet of Picus sp. We collected and analysed six dropping samples from anthills. We found 4 ant species Formica rufa, which was the most abundant prey, Formica sanguinea, Formica pratensis and also probably Formica polyctena.