The aim of this work was to identify cultivable bacteria and genetic variability of these bacteria from alkaline drainage water from bauxite residue sludge from aluminum production near Žiar nad Hronom and to describe their possible use in biotechnology. The brown and red mud were deposited to sludge during aluminum production by sintering method. The sludge currently belongs to recultivated environmental loads, although it remains a significant anthropogenic structure in the area. Drainage water, despite its high pH and heavy metal content, is home to a large microbial community. By cultivation analysis and MALDI-TOF analysis we identified several genus of microorganisms whose properties appear to be suitable for biotechnology, that means resistance to heavy metals and the ability to degrade aromatic hydrocarbons. Alkalotolerant microflora was consisted of bacteria species Bacillus benzoevorans, Alishewanella fetalis and Novosphingobium resinovorum.