The dissertation deals with the theoretical part of cross laminated timber (CLT). It is defined by its definition, key advantages, production process, economic comparison with traditional buildings and examples of cross laminated timber construction abroad. Further, in the theoretical part, the work is focused on a special type of cross laminated timber, namely CLT panels called Thoma Wood 100. Described is its definition, method of production and production, declared properties and design solutions. The practical part contains the methodology of measuring selected wood parameters for the production of standard CLT panels and Thoma wood 100. It is the measurement of the sound absorption of wood used for the production of these types of panels and their mutual comparison. Measurement of elastic module by non-destructive methods and their mutual comparison. The work includes the measurement of the sound insulation and impact sounds insulation of CLT panels of various compositions that were used as a construction element in the construction. The measured values are compared with the normalized values. As a result, recommendations are made to ensure acoustic comfort in wood buildings from CLT panels. Further, the Importance of Using Thoma Wood 100. Part of the dissertation is a program to calculate the sound insulation and impact sounds insulation of the dividing structures that was created in the framework of the research activity.