Nowadays poplars present hardwood, which belongs to fast growing, high biomass crops, high sprouting and adaptable to multiple environmental stressors. This fast growing woody plants are suitable for plantation. Genus populus are typical with it spontaneous hybridization. Current breeding strategies created tens poplar hybrids which are planted all around the world, but i tis necessery to propagate individuals with selected genotype. The aim of this dissertation work was compare two methods of vegetative propagation of hybrid poplar 'T-14' Populus tremula × (Populus × canescens) on leaf and wood level. Control group propagated by root cuttings was compared with plants propagated from in vitro tissue culture, we assessed leaf performance for any differences in anatomical, vascular and nanomechanical traits properties of the tracheary element cell walls and main content of wood components. The examiden vegetative propagation techniques had no significant effect on leaf and wood vascular traits. We found differences between the micropropagated plants and the plants propagated from root cuttings in leaf growth traits in favor of in vitro plants, which indicates higher transpiration. Atomic force microscopy in mode PeakForce QNM showed higher value of dissipation for in vitro plants, which can mean the higher ability of the cell walls to absorb more energy suggests a higher external damping capacity. Micropropagated plants had higher value content of cellulose to compare with root cuttings which nad higher value content of hemicellulose and their saccharides of which they are composed (xylose and arabinose). The principal component analysis (PCA) of leaves showed that micropropagated plants was more homogeneous than root cuttings, while PCA of secondary xylem showed opposite trend.