The main aim of the thesis is to research new and alternative methods in pest managemet against damage caused by pine weevil (Hylobius abietis L.). To achieve this main objective, partial objective were set: (1) assessment of biological methods of protection of coniferous seedlings against damage caused by pine weevil, (2) assessment of mechanical methods of protection of coniferous seedlings against damage caused by pine weevil and (3) revision of STN 48 2712 Forest protection against weevils and bark beetles on seedlings, which included new methods of protection of coniferous seedlings. Research with biological methods in forest protection began in 2013. We tested 3 spesies of entomopathogenic fungi Beauveria bassiana, Metarhizium anisopliae a Isaria fumosorosea. There was a series of five experiments in which the strain of B. bassiana isolated from a dead specimen caught from nature had the best results against tha large pine weevil. We focused on this strain, and testet it various concentrations (105,106,107,108 conidia/ml of water suspension). The best results were achieved with the concentration of 108 conidia/ml, which reached 100% mortality of adult pine weevil in the shortest time (13.5 days in average). In 2014 started experiments with new method of mechanical protection of coniferous seedlings in Slovakia – wax treated seedlings. The results showed that the wax can protect the seedlings against damage caused by pine weevil. But in the case of higher abundace of pine weevil adults, and in closed conditions, without access to another source of food, they can overcome this barrier. In 2016, we compared the ability of 2 agents, glue (Vermifix) and Pellacol to protect the seedlings. The results showed that the glue can successfully protect the seedlings and adults avoided them even after the death of all untreated seedlings. Still, we have encountered the deaths of several individuals treated with glue, which may have been due to the phytotoxicity of it. In 2016, new STN 48 2712 came out and fully replaced the old version of 1997. The main changes concern the modification and completion of some terms and definitions, especially as regards the waxing and alignment of STN with legislation.